Post by Moderator on Nov 9, 2003 17:42:58 GMT -5
From: fawnmarie42 (Original Message) Sent: 7/2/2003 9:37 PM
As some of you may know, I've begun work in my "Titty Opus" (which is basically my complete and written up findings on breast development and BE).
Here's something I ran across and thought I would share. I am going to incorporate it into the finished work, which will be posted on a website I'm working on (along with other herbal health/beauty info).
Note that red clover (isn't that what our very-busted Pammy uses) fits both estrogen receptors (and stimulates them) AND progesterone receptors (and blocks them).
Also note that ER (estrogen) receptors in breast cancer cells are the same receptors in normal cells and stroma. We can assume that the following info applies to healthy tissue as well.
Fawn
Estrogen and progestin bioactivity of foods, herbs, and spices.
Proc Soc Exp Biol Med 1998 Mar;217(3):369-78 (ISSN: 0037-9727)
Zava DT; Dollbaum CM; Blen M
Aeron Biotechnology, San Leandro, California 94577, USA.
In this study we report on the content and bioactivity of plant (phyto) estrogens and progestins in various foods, herbs, and spices, before and after human consumption. Over 150 herbs traditionally used by herbalists for treating a variety of health problems were extracted and tested for their relative capacity to compete with estradiol and progesterone binding to intracellular receptors for progesterone (PR) and estradiol (ER) in intact human breast cancer cells. The six highest ER-binding herbs that are commonly consumed were soy, licorice, red clover, thyme, tumeric, hops, and verbena. The six highest PR-binding herbs and spices commonly consumed were oregano, verbena, tumeric, thyme, red clover and damiana. Some of the herbs and spices found to contain high phytoestrogens and phytoprogestins were further tested for bioactivity based on their ability to regulate cell growth rate in ER (+) and ER (-) breast cancer cell lines and to induce or inhibit the synthesis of alkaline phosphatase, an end product of progesterone action, in PR (+) cells. In general, we found that ER-binding herbal extracts were agonists, much like estradiol, whereas PR-binding extracts, were neutral or antagonists. The bioavailability of phytoestrogens and phytoprogestins in vivo were studied by quantitating the ER-binding and PR-binding capacity of saliva following consumption of soy milk, exogenous progesterone, medroxyprogesterone acetate, or wild mexican yam products containing diosgenin. Soy milk caused a dramatic increase in saliva ER-binding components without a concomitant rise in estradiol. Consumption of PR-binding herbs increased the progestin activity of saliva, but there were marked differences in bioactivity. In summary, we have demonstrated that many of the commonly consumed foods, herbs, and spices contain phytoestrogens and phytoprogestins that act as agonists and antagonists in vivo.
Evaluation of the estrogenic effects of legume extracts containing phytoestrogens.
J Agric Food Chem 2003 Apr 9;51(8):2193-9 (ISSN: 0021-8561)
Boue SM; Wiese TE; Nehls S; Burow ME; Elliott S; Carter-Wientjes CH; Shih BY; McLachlan JA; Cleveland TE
Agricultural Research Service, Southern Regional Research Center, U.S. Department of Agriculture, New Orleans, Louisiana 70179, USA. sboue@nola.srrc.usda.gov.
Seven legume extracts containing phytoestrogens were analyzed for estrogenic activity. Methanol extracts were prepared from soybean (Glycine max L.), green bean (Phaseolus vulgaris L.), alfalfa sprout (Medicago sativa L.), mung bean sprout (Vigna radiata L.), kudzu root (Pueraria lobata L.), and red clover blossom and red clover sprout (Trifolium pratense L.). Extracts of kudzu root and red clover blossom showed significant competitive binding to estrogen receptor beta (ERbeta). Estrogenic activity was determined using an estrogen-dependent MCF-7 breast cancer cell proliferation assay. Kudzu root, red clover blossom and sprout, mung bean sprout, and alfalfa sprout extracts displayed increased cell proliferation above levels observed with estradiol. The pure estrogen antagonist, ICI 182,780, suppressed cell proliferation induced by the extracts, suggesting an ER-related signaling pathway was involved. The ER subtype-selective activities of legume extracts were examined using transiently transfected human embryonic kidney (HEK 293) cells. All seven of the extracts exhibited preferential agonist activity toward ERbeta. Using HPLC to collect fractions and MCF-7 cell proliferation, the active components in kudzu root extract were determined to be the isoflavones puerarin, daidzin, genistin, daidzein, and genistein. These results show that several legumes are a source of phytoestrogens with high levels of estrogenic activity.
As some of you may know, I've begun work in my "Titty Opus" (which is basically my complete and written up findings on breast development and BE).
Here's something I ran across and thought I would share. I am going to incorporate it into the finished work, which will be posted on a website I'm working on (along with other herbal health/beauty info).
Note that red clover (isn't that what our very-busted Pammy uses) fits both estrogen receptors (and stimulates them) AND progesterone receptors (and blocks them).
Also note that ER (estrogen) receptors in breast cancer cells are the same receptors in normal cells and stroma. We can assume that the following info applies to healthy tissue as well.
Fawn
Estrogen and progestin bioactivity of foods, herbs, and spices.
Proc Soc Exp Biol Med 1998 Mar;217(3):369-78 (ISSN: 0037-9727)
Zava DT; Dollbaum CM; Blen M
Aeron Biotechnology, San Leandro, California 94577, USA.
In this study we report on the content and bioactivity of plant (phyto) estrogens and progestins in various foods, herbs, and spices, before and after human consumption. Over 150 herbs traditionally used by herbalists for treating a variety of health problems were extracted and tested for their relative capacity to compete with estradiol and progesterone binding to intracellular receptors for progesterone (PR) and estradiol (ER) in intact human breast cancer cells. The six highest ER-binding herbs that are commonly consumed were soy, licorice, red clover, thyme, tumeric, hops, and verbena. The six highest PR-binding herbs and spices commonly consumed were oregano, verbena, tumeric, thyme, red clover and damiana. Some of the herbs and spices found to contain high phytoestrogens and phytoprogestins were further tested for bioactivity based on their ability to regulate cell growth rate in ER (+) and ER (-) breast cancer cell lines and to induce or inhibit the synthesis of alkaline phosphatase, an end product of progesterone action, in PR (+) cells. In general, we found that ER-binding herbal extracts were agonists, much like estradiol, whereas PR-binding extracts, were neutral or antagonists. The bioavailability of phytoestrogens and phytoprogestins in vivo were studied by quantitating the ER-binding and PR-binding capacity of saliva following consumption of soy milk, exogenous progesterone, medroxyprogesterone acetate, or wild mexican yam products containing diosgenin. Soy milk caused a dramatic increase in saliva ER-binding components without a concomitant rise in estradiol. Consumption of PR-binding herbs increased the progestin activity of saliva, but there were marked differences in bioactivity. In summary, we have demonstrated that many of the commonly consumed foods, herbs, and spices contain phytoestrogens and phytoprogestins that act as agonists and antagonists in vivo.
Evaluation of the estrogenic effects of legume extracts containing phytoestrogens.
J Agric Food Chem 2003 Apr 9;51(8):2193-9 (ISSN: 0021-8561)
Boue SM; Wiese TE; Nehls S; Burow ME; Elliott S; Carter-Wientjes CH; Shih BY; McLachlan JA; Cleveland TE
Agricultural Research Service, Southern Regional Research Center, U.S. Department of Agriculture, New Orleans, Louisiana 70179, USA. sboue@nola.srrc.usda.gov.
Seven legume extracts containing phytoestrogens were analyzed for estrogenic activity. Methanol extracts were prepared from soybean (Glycine max L.), green bean (Phaseolus vulgaris L.), alfalfa sprout (Medicago sativa L.), mung bean sprout (Vigna radiata L.), kudzu root (Pueraria lobata L.), and red clover blossom and red clover sprout (Trifolium pratense L.). Extracts of kudzu root and red clover blossom showed significant competitive binding to estrogen receptor beta (ERbeta). Estrogenic activity was determined using an estrogen-dependent MCF-7 breast cancer cell proliferation assay. Kudzu root, red clover blossom and sprout, mung bean sprout, and alfalfa sprout extracts displayed increased cell proliferation above levels observed with estradiol. The pure estrogen antagonist, ICI 182,780, suppressed cell proliferation induced by the extracts, suggesting an ER-related signaling pathway was involved. The ER subtype-selective activities of legume extracts were examined using transiently transfected human embryonic kidney (HEK 293) cells. All seven of the extracts exhibited preferential agonist activity toward ERbeta. Using HPLC to collect fractions and MCF-7 cell proliferation, the active components in kudzu root extract were determined to be the isoflavones puerarin, daidzin, genistin, daidzein, and genistein. These results show that several legumes are a source of phytoestrogens with high levels of estrogenic activity.
